Author links open the overlay panel. Numbers correspond to the affiliation list which can be exposed by using the show more link. Available online. Cells were treated with capillin to examine both the anti-proliferative and pro-apoptotic effects, as well as the molecular mechanism underlying these effects. Changes in cell proliferation, membrane permeability, macromolecular synthesis, glutathione (GSH cell cycle and programmed cell death were evaluated. The thermal degradation of poly(vinyl bromide) (PVB poly(vinyl chloride) (PVC poly(vinyl alcohol) (PVA poly(vinyl acetate) (PVAc poly(vinyl fluoride) (PVF poly(vinylidene chloride) (PVC2 and poly(vinylidene fluoride) (PVF2) has been studied by direct pyrolysismass spectrometry (DP-MS) and flash pyrolysisgas chromatographymass spectrometry techniques. These findings suggest that capillin has cytotoxic activity and can induce apoptosis in human tumour cell lines. The overall thermal degradation process depends on the prevailing decomposition reaction in each polymer; therefore, different behaviors are observed. The thermal degradation of polyacetylene (PA) has also been studied and found important for the elucidation of the thermal decomposition mechanism of the title polymers.
Capillin (1microM-10microM) inhibited cell proliferation and decreased macromolecular synthesis simultaneously, in a dose- and time-dependent manner. Co-incubation with L-buthionine sulfoximine (L-BSO) augmented the efficacy of capillin. Capillin modulated GSH levels, accumulated cells in the SG2/M-phase of the cell cycle and induced cell death and DNA fragmentation, as indicated by flow cytometry, fluorescence microscopy and DNA fragmentation assay.
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